Product Name:
Human Interleukin 17 (IL-17) ELISA Kit
Cat.#:
JL19255
Regulatory Status:
Alternative:
IL17A; CTLA8; IL-17A; Cytotoxic T-Lymphocyte-Associated Protein 8
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
1.56-100pg/mL
Sensitivity:
0.41pg/mL
Specificity:
This kit recognizes Human IL-17 in samples. No significant cross-reactivity or interference between Human IL-17 and analogues was observed.
Sample Type:
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Procedure:

Research Area:
Immunology
Product Datasheets
| IL-17
Interleukin-17 (IL-17) is a family of pro-inflammatory cytokines. They are produced by a group of T helper cell known as T helper 17 cell in response to their stimulation with IL-23. The most notable role of IL-17 is its involvement in inducing and mediating proinflammatory responses. IL-17 family has been linked to many immune-related/autoimmune diseases including rheumatoid arthritis, asthma, lupus, allograft rejection, anti-tumour immunity and recently psoriasis, multiple sclerosis, and intracerebral hemorrhage.| Typical data
As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.Concentration (pg/mL) | 100 | 50 | 25 | 12.5 | 6.25 | 3.12 | 1.56 | 0 |
OD | 2.04 | 1.49 | 0.97 | 0.63 | 0.41 | 0.29 | 0.24 | 0.09 |
Corrected OD | 1.95 | 1.4 | 0.88 | 0.54 | 0.32 | 0.2 | 0.15 | - |
| Repeatability
Intra-assay Precision (Precision within an assay):CV% < 10%; Inter-assay Precision (Precision between assays):CV% < 10%| Recovery
The recovery of Human IL-17 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.Sample Type | Range(%) | Average Recovery(%) |
Serum(n=8) | 84-101 | 96 |
Plasma(n=8) | 92-105 | 102 |
Cell Culture Supernates(n=8) | 96-108 | 105 |
| Linearity
Samples were spiked with high concentrations of Human IL-17 and diluted with Universal Diluent to produce samples with values within the range of the assay.Serum(n=4) | Plasma(n=4) | Cell Culture Supernates(n=4) | ||
1 : 2 | Range (%) | 84-95 | 88-96 | 90-110 |
Average (%) | 91 | 93 | 96 | |
1 : 4 | Range (%) | 89-103 | 87-108 | 105-115 |
Average (%) | 94 | 98 | 108 |






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48T


280 / 280
96T


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